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As the search model. The wild type SHP2 and D61G, E139D, Y279C and Q506P mutant structures were determined by molecular replacement, using the structure of the N308D SHP2 mutant as a search model. Following the initial rigid body refinement, interactive cycles of model building and refinement were performed by using COOT [40] and Buster-TNT [41] software. Special attention was paid to the mutation
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